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elution buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs elution buffer
    Elution Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+h+elution+buffer/RNase+H/pm41233305-440-10-29
    Average 99 stars, based on 5390 article reviews
    elution buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA Airn
    Article Snippet: Beads were resuspended in 100μL RNase H Elution Buffer and treated with 2μL RNase H (NEB, cat #: M0297) for 10 min at room temperature.

    Article Title: Ornate, large, extremophilic (OLE) RNA forms a kink turn necessary for OapC protein recognition and RNA function.
    Article Snippet: The beads were then resuspended in 50 μl of RNase H elution buffer containing 10 U RNase H (New England BioLabs).

    Incubation:

    Article Title: Aberrant splicing exonizes C9ORF72 repeat expansion in ALS/FTD
    Article Snippet: .. All washing steps were performed at 58 °C for 5 min. After being washed twice in 200 μl RNase H Elution Buffer (50 mM Tris-HCl pH 7.5, 75 mM NaCl, and 3 mM MgCl 2 ; freshly supplemented with 0.125% N-lauroylsarcosine, 0.025% sodium deoxycholate, and 2.5 mM TCEP), RNAs were eluted by mixing the beads with 21 μl RNase H Elution Buffer and 4 μl RNase H (New England Biolabs) and incubated at 37 °C for 30 min while shaking. ..

    Article Title: Aberrant splicing exonizes C9ORF72 repeat expansion in ALS/FTD
    Article Snippet: .. All washing were performed at 58 °C for 3-10 min. After being washed twice in 200 μl RNase H Elution Buffer (50 mM Tris-HCl pH 7.5, 75 mM NaCl, and 3 mM MgCl 2 ; just before use, add 0.125% N-lauroylsarcosine, 0.025% sodium deoxycholate, and 2.5 mM TCEP), RNAs were eluted by mixing the beads with 21μl RNase H Elution Buffer and 4 μl RNase H (New England Biolabs) and incubated at 37 °C for 30 min while shaking. ..

    Article Title: A viral lncRNA tethers HSV-1 genomes at the nuclear periphery to establish viral latency.
    Article Snippet: .. To elute, 55 μL of RNase H Elution Buffer and 7.5 μL of RNase H (NEB, M0297) were added to each sample and incubated at 37°C for 30 min with shaking. ..

    Article Title: Aberrant splicing exonizes C9orf72 repeat expansion in ALS/FTD.
    Article Snippet: A nucleotide repeat expansion (NRE) (GGGGCC)n within the first annotated intron of the C9orf72 (C9) gene is a common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).. While previous studies have shown that C9 NRE produces several toxic dipeptide repeat (DPR) proteins, the mechanism by which an intronic RNA segment can access the cytoplasmic translation machinery remains unclear.. By selectively capturing and sequencing NRE-containing RNAs (NRE-capture-seq) from patient-derived fibroblasts and neurons, we found that, in contrast to previous models, C9 NRE is retained as part of an extended exon 1 due to the usage of various downstream alternative 5′ splice sites.

    Saline:

    Article Title: LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs.
    Article Snippet: Article LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs



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